Fig. 3

Lineage-defining SNPs and insertion of lineage B.1.214.2. a Genomes belonging to the B.1.214 clade are defined inside the dashed-lined box. SNPs that differentiate from the reference (GenBank accession MN908947) and are found in at least two B.1.214 sequences are shown in the condensed SNP alignment. Nucleotides that are shared with the reference strain are shown in grey, while changes from the reference are colored and ambiguities are shown in dark grey. The phylogeny (branch lengths in the number of mutations) on the right shows the relationships between depicted genomes and was rooted on the reference sequence. The long branch defining B.214 is labeled as ‘B.1.214’. Greek letters represent groups of sequences with note-worthy mutational profiles. α = pangolin definition B.1.214.2 (Q414K, N450K, Insertion, and orf1a (50%) and orf3a deletions), β = B.1.214.1 with insertion sequence but missing critical Q414K and N450K, δ = B.1.214 with insertion, Q414K, N450K- possible misclassification by pangolin, ε = Belgian nursing home outbreak; γ = B.1.214.2 that lack insertion sequence but contain Q414K, N450K. b Venn diagram separated by different lineage-defining mutations. Greek letters represent the same sequences as described above. α represents B.1.214.2 both with and without the orf1a deletion