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Fig. 5 | BMC Infectious Diseases

Fig. 5

From: Advancing schistosomiasis surveillance: standardization and application of an environmental DNA (eDNA)-based approach for detecting Schistosoma mansoni in Brazil

Fig. 5

Loop-mediated isothermal amplification (LAMP) and Quantitative PCR (qPCR) assays to detect Schistosoma mansoni detection in Giru district, Joaíma, Minas Gerais, Brazil. LAMP amplifications from collection points were visualized on silver-stained 6% polyacrylamide gels (A) and by color change with SYBR Green I by the naked eye (B) and by exposure to ultraviolet light (C). 100 pb: marker (Promega); W: blank samples; A, B, C: samples from respective collection points. (D) The graph depicts the change in fluorescence intensity (ΔRn) throughout qPCR amplification cycles for all collection points. For all graphs, negative controls and blank samples are shown in gray, and positive controls in pink. Graphs were generated using the ggplot2 package (v. 3.5.1) in the R statistical software (v. 4.4.0) (https://www.r-project.org/)

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BMC Infectious Diseases

ISSN: 1471-2334

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